Journal: International Journal of Oral Science

Article Title: Implantation awakens peri-implant osteogenic potential via Snx5-EGFR axis-mediated mechanical transduction

doi: 10.1038/s41368-025-00423-2

Figure Lengend Snippet: Dental implant placement maintains alveolar bone height and promotes osteogenesis, associated with downregulation of Snx5 in peri-implant regions. a Schematic diagram illustrating the effects of natural socket healing following tooth extraction and implant placement on alveolar bone height. b Representative radiographic images and quantitative analysis of bone resorption following natural healing or implant placement ( n = 20). c DEGs were identified by integrating three transcriptomic datasets: (1) LepR⁺ cells from peri-implant and control regions ( GSM7109973 and GSM7498409 ); (2) bulk RNA-seq data from peri-implant bone tissue (PRJNA924055 and GSE154748 ); and (3) MSCs under mechanical load in vitro ( GSE112122 ). d Venn diagram showing 11 overlapping DEGs across the three datasets, with Snx5 being the only gene consistently downregulated. e Immunofluorescence staining and quantification of Snx5 (green), LepR-tdTomato (red), and DAPI (blue) in peri-defect or peri-implant tissues from Sham and Imp groups. Overview and magnified views are shown on the left. Yellow dashed boxes indicate zoomed regions ( n = 5). Scale bars: 100 μm. f Immunofluorescence staining and quantification of Osx (green), LepR-tdTomato (red), and DAPI (blue) in peri-defect or peri-implant tissues from Sham and Imp groups. Overview and magnified views are shown on the left. Yellow dashed boxes indicate zoomed regions ( n = 5). Scale bars: 100 μm. g Correlation analysis between the number of Snx5⁺ LepR⁺ tdTomato⁺ cells and Osx⁺ LepR⁺ tdTomato⁺ cells ( n = 10). RNA-seq RNA sequencing, DEGs differentially expressed genes, BMSCs bone marrow stromal cells, Imp implant, Data are presented as mean ± SD. ns not significant, * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.000 1. Statistical analysis: two-tailed Student’s t-test for panels b , e and f ; linear regression for g with the coefficient of determination ( R ²) shown

Article Snippet: The library preparation was carried out according to the instructions provided with the RNA-Seq Library Preparation Kit (NR606, Vazyme, China).

Techniques: Extraction, Control, RNA Sequencing, In Vitro, Immunofluorescence, Staining, Two Tailed Test